STExplore
STexplore is an integrated web server for comprehensive spatial transcriptomics analysis of user-uploaded data(ST data and scRNA-seq data). It builds a comprehensive analysis pipeline that includes preprocessing, integration with scRNA-seq, cell-level analysis, gene-level analysis, cellular communication.
Preprocessing
Preprocessing encompasses a suite of essential steps including quality control, filtering, normalization, and the selection of highly variable genes.
Integration
Given the inherent limitations of ST technologies, we harnessed the power of scRNA-seq data integration to address these constraints.
Cell level analysis
Cell analysis methods attempt to explain the heterogeneity in the data based on a categorization of spots into groups, and the relationships between clusters
Gene level analysis
DEG, ssGSEA and SVG investigate molecular signals.These methods use cellular/spatial heterogeneity as a context for understanding gene expression.
Cell cell communication
CCC refers to the network of moleculars interacted between cells, capable of providing a system-level hypotheses of intercellular crosstalk in vivo.
